The
Oldest Fossils
Stromatolites
are not only Earth's oldest of fossils, but are intriguing
in that they are our singular
visual portal (except for phylogenetic
determination of conserved nucleic acid sequences and some
subtle molecular fossils) into deep time on earth, the emergence
of
life,
and the evolving of the beautiful forms of life of modern time.
A small piece of stromatolites encodes biological activity
perhaps
spanning thousands of years. In broad terms, stromatolites are
fossil evidence of the prokaryotic life that remains today,
as it has always
been, the preponderance of biomass in the biosphere. For those
that subscribe
to the theory of the living earth, it is the prokaryotes that
maintain the homeostasis of the earth, rendering the biosphere
habitable
for all other life. They maintain and recycle the atomic ingredients
of which proteins, the essence of life, are made, including
oxygen,
nitrogen and carbon. We humans are, in simple terms, bags of water
filled with proteins and prokaryotic bacteria (the bacteria
in your
body outnumber the cells in your body about 10 to 1). We humans
have descended from organisms that adapted to living in a
prokaryotic
world, and we humans retain (conserved in evolutionary terms) in
our Eukaraotic mitochondria the cellular machinery to power
our cells that
we inherited (i.e., Endosymbiosis)
from the prokaryotes of deep time on earth.
Defining Stromatolites
Scientists
disagree on how to define stromatolites. A common definition
goes something like: A lamiated rock formed by the
growth of blue-green
algae (i.e., cyanobacteria)". This definition is, in fact,
such a gross oversimplification as be scientifically useless.
It does contain a modicum of truth, however, in that the largest
volume of stromatolitic formations was likely formed by biogenic
processes involving photosynthetic cyanobacteria. Cyanobacteria’s
metabolic byproduct, oxygen, rusted the earth, pumped enormous
oxygen poison to them into earth’s atmosphere, and in so
doing paved the way for aerobic-based life to emerge and diversify;
cyanobacteria’s contributions to life led to their own
prodigious decline.
Stromatolites
and their close cousins the thrombolites, are rock-like buildups
of microbial mats that form in limestone- or dolostone-forming
environments. Together with oncoids (formerly called "algal
biscuits" or "Girvanella"), they typically form
by the baffling, trapping, and precipitation of particles by
communities
of microorganisms such as bacteria and algae. In some cases,
they can form inorganically, for example when seawaters are oversaturated
with certains chemicals resulting in precipitation. Stromatolites
are defined as laminated accretionary structures that have synoptic
relief (i.e., they stick up above the seafloor). Stromatolite-building
communities include the oldest known fossils,
dating back some 3.5 billion years when the environments of Earth
were too hostile to support life as we know it today. We can presume
that the microbial communities consisted of complex consortia
of species with diverse metabolic needs, and that competition
for resources and differing motility among them created the intricate
structures we observe in these ancient fossils. Microbial communities
diversified through time, with eukaryotic
organisms eventually
joining the mix.
Excluding
some exceedingly rare Precambrian fossils such as the Russian
White Sea Ediacaran
fauna, stromatolites and thare the only fossils encoding the
first 7/8th of the history of life on earth. They encode the
role that
ancient microorganisms played in the evolution of life on earth
and in shaping earth's environments. The fossil record of
stromatolites
is astonishingly extensive, spanning some four billion years
of geological history with the forming organisms possibly
having
occupied every conceivable environment that ever existed on earth.
Today, stromatolites are nearly extinct in marine environments,
living a precarious existence in only a few localities worldwide.
Modern stromatolites were first discovered in Shark Bay, Australia
in 1956, and throughout western Australia in both marine and
non-marine environments. New stromatolite localities have
continued to be
discovered in various places such as the Bahamas, the Indian
Ocean and Yellowstone National Park, to name but a few localities.
|
Precambrian
rock exposures within the United States |
Stromatolite Stratigraphy:
Stromatolites
occur throughout the world, but become diminishingly uncommon
in the Archaean. Earth’s violent surface,
floating on tectonic plates, is subjected to volcanism, subduction (into
the earth’s mantle), uplift, metamorphism, and enormous
erosion forces. Probably many Archaean rocks have succumbed
to
these many forces. So too have many Stromatolites from
the Proterozoic. Production of stromatolites has been relatively
minor during the
Phanerzoic Eon with its entirely different reef ecosystems.
What rock has not been lost may of course be buried deeply.
The figure
to the left shows Precambrian exposure within the United
States. The figure indicates the paucity of localities
where
finding the
oldest fossils exists. The north, central U.S. and Wisconsin
and Minnesota particularly have much old rock, and not
surprisingly, considerable Stromatolites and banded
iron.
| Domain:
Eubacteria
Phylum:
Cyanobacteria
Genus: Anabaena |
The
Three Domains of Life
As
previously noted, stromatolites are most often described as
biogenically-produced
structures formed by colonies of photosynthesizing
cyanobacteria. However, this is an enormous oversimplification
given that the weight of scientific evidence suggests
that all
three
domains of life (the Archaeans, Eubacteria, and Eukaryotes)
appeared in the Archaean Era, and thus the so-called microbial
mats would have
contained representatives among all three domains. Just how
and when the base of the tree of life split into the three main
branches
remains one of the most important questions in all of biology
and science, and is the source of constant scientific dispute.
Which of the prokaryotes came first, the Archaeans or the Eubacteria
remains unresolved, and a consensus has emerged that these
primitive
microorganisms laterally exchanged genes further confounding
attempts to validate what begat what during to course of
early evolution
on earth. Lateral gene transfer belies the concept of the single
common ancestor (see Woese, 1998). While formation by colonies
of cyanobacteria is probably the primary mechanism for formation
of surviving stromatolites in the deep time of the Archaean
and
half way through the Proterozoic, it is unlikely to have been
the only mechanism.
Recent
research (Gupta, 1998a, 1998b, and an extensive literature) indicates
the other prokaryotic and the most genetically diverse domain
of life, the Archaeans, evolved alongside each other and possibly
swapped genes with the Eubacteria. All prokaryotes (both Eubacteria
and the Archaeans reproduce by cell division (binary or multiple
fission) and, lacking sex, are essentially clones and among the
slowest evolving organisms. Moreover, some microfossils (actually,
putative ancient cellular remnants) indicate that primitive Eukaryotic
microorganisms may have appeared prior to 3.5 Bya. Thus, before
the end of the Archaean time some 2.5 Ba, all three domains of
life (Eubacteria, Archaea, and Eukaryotes) co-existed and were
likely already quite diverse. Some were autotrophs, some chemotrophs and some heterotrophs, and collectively they had a multiplicity
of metabolic processes from which to derive their energy, and
as they do today. Just as microorganisms were extremely diverse
in deep time, so were there a corresponding extreme diversity
of biogenic and chemical (abiogenic) mechanisms that are plausible
for the formation of laminar carbonate and other structures that
we call stromatolites, and the possible ancient cellular microstructions
they might contain. Ascribing all stromatolite formation in the
Archaean and Proeterozoic to cyanobacteria, as is often seen in
general literature, is incorrect.
Whether
or not stromatolites contain preserved cellular structures (microfossils)
also remains highly contencious, especially in older Arachean
rocks. Viewing these putative ancient cells requires special polishing
techniques and high magnification. Additionally, Molecular fossils
(or fingerprints) based on atom ratios in Archaean sediments is
highly contencious, but scientifically critical, since such subtle
molecular traces found in Australia has led to conjecture that
microorganisms with nuclei appeared before 3.8 Ba.
Milestones
in Evolutionary Biology - Bacteria, Archaea and Eukarya
Stromatolites
may hold the key to determining one of the most important questions
in evolutionary
biology, how and when the
tree of life branched into the three domains, the Bacteria,
Archaea and Eukarya. Of particular importance is when microorganisms
with
advanced photosynthetic metabolism first appeared, since
molecular phylogenetics suggests that all three domains would
have already
appeared and significant evolution must have already taken
place (Schopf, 2000 and Olson 2006). Equivalently important
is when
the eukaryotic nuclear genome became a chimera with contributions
from both the Archaean and Bacteria (Gupta, 1997). While there
is scientific consensus that phosynthetic cyanobacteria became
prodigious at some point in the Archaean, as evidenced by
first
the rusting of the earth and ultimately the oxygenation of
the atmosphere, just when that occurred remains both uncertain
and
contentious. More generally, stromatolitic laminate structures
themselves as well as the putative cellular structures and
molecular
signatures they sometimes contain have been and remain controversial,
since they can be explained by either biogenic or abiotic
processes
(Grotzinger, 1999). Further confounding the definition of what
is or nor not stromatolites is known processes for bacterially
mediated precipitation of minerals (Paerl, 2001).
Warrawoona
Group in Western Australia - a scientific dispute
The
putative stromatolites with microstructures resembling bacteria
from the extensive stromatolitic formations of the 3,430-million-year-old
Strelley Pool Chert within the Warrawoona Group in Western Australia
have been hotly debated ever since their discovery by Lowe (1980,
1983). Lowe (1994) later ascribed conical form genera to abiotic
evaporative precipitation, as did Grotzinger (1999), and Brasier
(2002) also found no support for the microfossils as biomarkers.
Whether microstructures within the Warrawoona Group stromatolites
are the imprints of ancient filamentous and possibly photosynthetic
microbes as argued by Schopf (1987, 1993) and Awramik (1992)
became
a heated debate that remains unresolved. A recent and extensive
study of seven distinct stromatolitic form genera by Allwood
(2006)
certainly lends support to proponents of biogenetic origins of
the chert, since the simultaneous set of forms is more difficult
to explain with known abiogenic processes. However, whether the
microstructures are fossil microbes remains unresolved. If they
are microbe fossils, there would still remain the critical question
of what type they are, archaea, cyanobacteria, another type
of
photosynthetic bacteria, chemosynthetic bacteria, or some combination
of these.
Gunflint
Chert - abundant preserved
cells
While
the Warrawoona Group fossil microbes remain
equivocal regarding biological origins, those of the 2 Bya
Gunflint Chert in Canada is not. Gunflint microfossils are
diverse, abundant, and resemble the form of modern bacteria
(Cloud, 1965a; Cloud 1965b; Knoll, 1978). However, these iron-metabolizing
organisms are no longer prevalent in modern seas that contain
miniscule concentrations of iron. Such iron rich cherts as
the
Gunflint dating between 1.8 and 2.1 Bya are actually quite
prevalent on earth, an indisputable metric of the iron richness
of the early
Archaean seas.
Schoff
and coworkers report unambiguous cyanbacteria microfossils from
the Archaean, 2.6 Bya Campbell Group in Cape Province, South Africa
(Altermann, 1995), and numerous Proterozoic sites, including the
stromatolitic black chert from the approximately 850 Mya Bitter
Springs Formation, Australia (House, 2000), the 680-790 Mya Min'yar
Formation of the southern Ural Mountains (Nyberg, 1984). Recently,
Schopf, perhaps the most outspoken proponent of the evidence of
cyanobacteria in the early Arachaean, listed 48 Archaean deposits
reported possibly containing biogenic stromatolites, of which
14 contain 40 morphotypes of putative microfossils, and 13 are
in the age range of 3.2 to 3.5 Bya (Schopf, 2006).
Other
researchers strongly disagree. For example, Blank (2002), based
on genome sequencing, posits that cyanobacteria may have originated
as late as 2.3 billion years ago. The phylogenetic tree based
on whole genomic DNA sequences show that cyanobacteria were one
of the last major lineages to diverge off the bacterial tree,
and were preceded by sulfur-oxidizing bacteria and sulfate-reducing
bacteria. This mirrors the changes in the geochemical record,
centered around 2.7 billion years ago. The hypothesis is consistent
with geology that finds isotopic fractionation of sulfur compounds
becomes large, followed by the sudden increase in oxygen in the
atmosphere and surface water environments at about 2.2 or 2.3
Ba.
Oxygenation
of the Atmosphere - a profound transformation of the bioshere
Regardless
of when the cyanobacteria appeared, it is widely accepted
that they comprised the predominant form of life on early earth
for some two billion years, and were responsible for the creation
of earth's atmospheric oxygen, consuming CO2 and releasing
O2
by photosynthetic metabolism. Creation of the modern atmosphere
is, of course, perhaps the most critical event in geological
history
that powered the Cambrian
Explosion and subsequent evolution
of the aerobic forms of life, including all animals (Ohno,
1997).
Microbial
Mats to Stromatolitic Structures
During Precambrian times,
bacterial mats formed extensive platforms for trapping and cementing
sediment. For photosynthetic bacteria,
depletion of carbon dioxide in the surrounding water could cause
precipitation of calcium carbonate adding to grains of sediment
that were then trapped within the sticky layers of mucilage (that
the cyanobacteria formed as a film providing protection from
ultraviolet
radiation). Precipitants and sediments together formed stromatolitic
layers that grew one upon another, with the living baterial
colonies
occupying the uppermost layer. Cyanobacteria are also capable
of directly precipitating calcium carbonate with minimal incorporation
of sediment within the structure. The bacteria could repeatedly
re-colonize the growing, hard sedimentary platform, forming
layer
upon layer in a cyclic repetitive process. The resulting successive
layering can assume a myriad of shapes dependent upon microorganism
and environment, and if left undisturbed by forces of nature
could form huge domes and flat laminar structures that grew
upward toward
the life-sustaining rays of the sun.
Stromatolites
are also variously described as being formed by algae that
are, in turn, assumed to be plants; this description
still persists in old textbooks and on the Internet, but is vary
incorrect. It is a holdover from a time that cyanobacteria
were
thought to be algae (and were called blue-green algae) and from
when algae were thought to be plants. Actually, cyanobacteria
are prokaryotic bacteria (domain of life Eubacteria), and "genomic" science
is sill debating whether eukaryotic, photosynthetic, and autotrophic
algae are plants or deserve a distinctive phylogenetic
grouping. Regardless, the eukaryotic algae did not appear until
about 1.5 Ba, some 2 billion years after stromatolites significantly
began forming. It is therefore likely that stromatolite formation
by algae was not significant until the Phanerozoic, or possibly
the Late Proterozoic.
Banded
Iron Formations (BIFS)
While
not always recognized as such, Banded Iron Formations
(BIFs) are another form of stromatolites, and again the cyanobacteria
are the heroes that provided the source of oxidants for BIF formation.
BIFs are massive, laterally extensive and globally distributed
chemical sediment deposits that consist primarily of Fe-bearing
minerals (iron oxides) and silica. Iron can occur naturally
in
two states. Reduced, or ferric iron, is soluble in water. In
the Archaean oceans, prodigious ferric iron was released
from the
Earth's interior. The presence of free oxygen in the oceans
would
have oxidized the reduced (soluble ferrous) iron in solution
to
form oxidized (insoluble ferric) iron, which precipitated as
iron oxide. Thus, banded iron layers are the result of
oxygen released
by photosynthetic organisms combining with dissolved iron in
Earth's oceans to form insoluble iron oxides. The banding
is assumed to
result from cyclic peaks in oxygen production. It is unclear
whether these were seasonal or followed some other cycle.
It is assumed
that initially the earth started out with vast amounts of iron
dissolved in the world's seas. BIFs occur in the geologic
record
from about 3.8 Bya (Isua, West Greenland) to about 1.8 Bya
with a maximal abundance at about 2.5 Bya, and a reoccurrence
in Neoproterozoic
time (from about 0.8 and 0.6 Bya). The scientific literature
commonly attributes the disappearance of BIFs to the
fact
that deep oceans
became oxidized at 1.8 Bya (Cloud, 1972); their formation ostensibly
required anoxic deep waters to deliver hydrothermally
derived
Fe2+ to locations where deposition took place. Konhauser (2002),
however, provides evidence of an alternative mechanism for
BIF
generation by organisms that directly oxidize Fe2+ as an energy
source. He argues that the bacterial genera Gallionella and
Chromatium
use such metabolism and that both are likely to have existed
in Precambrian oceans. Interestingly, it is estimated that
the amount
of oxygen locked up in earth's BIFs is some 10 times the amount
contained in the atmosphere.
Recent
research supports the hypothesis that stromatolite form diversity
increased through the Paleoproterozoic, reached a maximum in the
Mesaproterozoic at about 1.5 Bya that persisted to about 700 Ma,
and steadily declined to several taxa by the Precambrian-Cambrian
boundary (Olcott, et. al., and Wray, 1996). This is in contrast
to previous data indicating a steep decline at 2 Bya that now
appears from the data to be an artifact of 50% of all stromatolites
coming from a single basin and author; thus, this decline was
in volume. When this regional anomaly is removed, the steepest
decline in forms appears to have occurred in the Cambrian. By
normalizing stromatolite forms with volume of preserved carbonate
rock, the authors posit that the steepest decline in stromatolite
form diversity occured in the late Neoproterozoic, and culminated
in the Lower Cambrian, coincident with the widespread appearance
of macroscopic metazoa and significant bioturbation (i.e., activity
of bottom-living animals that keeps sediments oxygenated and homogenous).
Conversely, stromatolites indirectly support the hypothesis that
the diversification of major animal phyla occurred between 1 and
1.2 Ba (Wray, 1996). Since laminated sediments are a sign of oxygen
depletion in the bottom zone of the sea, bioturbation would inhibit
the building of stromatolitic structures. This research seems
consistent with evolutionary theory that would anticipate diversification
of stromatolite forming taxa due to selective pressure from other
organisms that were emerging and themselves diversifying.
We
will likely have no more than a sketchy understanding of the paleoenvironments
in which stromatolites were formed in the deep Precambrian time,
and only an incomplete understanding of the environments in the
Paleozoic. Sound conjecture is possible if we examine the now
rare environments that support stromatolitic growth during modern
times. Cyanobacteria are found to be a primary organism in the
formation of modern microbial carbonates. These prokaryotic bacteria
(slang name is blue-green algae owning to pigmentation involved
in photosynthesis) are now only found in areas where there is
reduced grazing and burrowing by other organisms, and a low occurrence
of macro-algae and plants. Environments where modern stromatolites
are found typically are hypersaline, but also include areas of
high alkalinity, low nutrients, high or low temperatures, and
strong wave or current actions. The obvious pattern emerges that
modern stromatolites tend to exist in areas that most other life
forms consider less desirable or possibly intolerable. Thus, organisms
producing modern stromatolites are generally limited to areas
where organisms with which they have to compete and/or organisms
that might use them for nutrients are not prevalent.
Also
see: Stromatolite
References Evolution
of Multicellularity Endosymbiosis
Leads to Mitochondria Endosymbiosis
Leads to Chloroplasts Museum Cambrian Explosion Fossils |